At that time, the early indications had been dismissed as debris, nevertheless the retrospective analysis demonstrated shared qualities With all the problematic cells in tradition. Tracing the infection served the lab establish supplemental laboratory processes for screening and defense of assay-vital cell strains.
Whether or not you’re a novice or an expert, discover our resources to advance your research and know-how in flow cytometry.
Merge acoustic focusing know-how with superior-parameter analysis for versatile spectral unmixing and traditional workflows.
Uncover new insights into your experimental facts by visualizing populations working with put together fluorescence and morphology data. Our application interface wizards empower downsampling, concatenation (merged data files) and dimensionality instruments that happen to be constructed in to the software for an intuitive, streamlined facts Investigation encounter.
"An image just isn't quite truly worth one thousand dots, but it actually really helps to see illustrations or photos. It is excellent to view illustrations or photos that correlate with staining. By way of example, AnnexinV-positive cells which might be morphologically various than nutritious cells, or CD14+ monocytes which can be much larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a seasoned person has gated singlets confidently. Following evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of greater than 4% aggregates.
Optimizing gating strategy. Even strong guide singlet gating is mistake-vulnerable and remains a subjective decision place in Practically all flow cytometry assays. Imaging can be utilized to verify and adjust gates to incorporate only solitary cells of curiosity.
Our experts will design panels to suit your needs as repeatedly you would like—totally free, no product buy required.
The Attune NxT cytometer will be the trustworthy workhorse instrument your lab can depend upon for reliable and reliable effectiveness. The Attune CytPix design encompasses a higher-speed brightfield digicam and AI-pushed graphic Evaluation program, with the latest updates which includes equipment Understanding instruments and an intuitive person interface, enabling here Increased knowledge precision, precision, and speedier insights into sample biology.
People can check out event illustrations or photos in actual-time to substantiate sample good quality, optimize protocols, and file the morphology of each and every mobile population discovered for analysis. Imaging seize also Gains from acoustic focusing, which really helps to position the cells in order that a sharp, centered impression is attained.
AI-driven automated picture Examination Using the Attune Cytometric Application translates celebration features into distinctive morphology parameters that could be combined with common fluorescence and scatter parameters to reveal hugely reproducible population variations and distinctive subsets.
Software note: Automated impression Examination minimizes user-to-user variability in flow cytometry gating approaches
Leverage the power of advanced technological innovation and innovation to speed up your discoveries. Trust during the Attune Flow Cytometer spouse and children to deliver the trustworthiness, velocity, and accuracy that established your work apart.
Adaptable payment approach from Agilent Economical Alternatives let you receive the important instrumentation you'll need for rapid challenge, or to improve your lab.
At the center of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which utilizes audio waves to meticulously align cells inside the flow mobile. This progressive method surpasses classic hydrodynamic approaches, leading to a lot quicker processing speeds, Excellent facts high-quality, and increased sample throughput.
The Attune Xenith cytometer is really a higher-parameter spectral analyzer, furnishing the two spectral unmixing and traditional flow capabilities for complete and adaptable mobile Examination.
Agilent Price Guarantee guarantees you at the least ten years of instrument use with the day you buy. Otherwise, We are going to credit history you Together with the residual worth of the technique toward an upgraded product.
In this article, we present that by gating tactics working with image-based quantitative parameters can distinguish interacting cells from coincident functions to more precisely examine interacting cells.