Facts-enriched characterization of mobile populations. Morphological details from pictures can add for the richness of flow cytometry details. As an example, the figure reveals an otherwise regular apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in Every single inhabitants to expose morphologically distinct options. These insights couldn't are actually obtained from multiplex staining alone.
As samples are acquired within the Attune CytPix Flow Cytometer, the higher-speed brightfield camera captures and retailers images of detected gatherings in a rate as many as 6,000 visuals for each next, depending on the flow charge and image size. For larger adaptability, Attune Cytometric Application lets you modify the graphic capture frequency as necessary and select a specific gate for picture capture, if necessary.
Incorporate acoustic concentrating technological innovation with superior-parameter Examination for versatile spectral unmixing and traditional workflows.
Find new insights into your experimental data by visualizing populations utilizing mixed fluorescence and morphology info. Our application interface wizards allow downsampling, concatenation (merged documents) and dimensionality tools which have been built into your software for an intuitive, streamlined knowledge Examination encounter.
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In this article, a highly skilled consumer has gated singlets confidently. Right after analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of more than four% aggregates.
Optimizing gating tactic. Even robust guide singlet gating is error-susceptible and continues to be a subjective decision position in Virtually all flow cytometry assays. Imaging can be used to substantiate and modify gates to incorporate only one cells of curiosity.
Our researchers website will layout panels to suit your needs as over and over you require—gratis, no product buy wanted.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can rely on for dependable and trustworthy performance. The Attune CytPix model encompasses a significant-velocity brightfield camera and AI-driven image analysis computer software, with modern updates like machine learning equipment and an intuitive person interface, enabling Increased info precision, accuracy, and more quickly insights into sample biology.
Most likely most significantly, these occasions include cells of Obviously unique phenotypes which could lead on to incorrect conclusions relating to double optimistic activities (particularly in rare populations).
Sound waves enable exact mobile alignment, resulting in exceptional data quality and ~10X more quickly sample flow rates.
Software note: Automatic picture Investigation reduces person-to-person variability in flow cytometry gating tactics
Constructed-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate predicted inhabitants resolution in lysed entire human blood. User can view events from Each and every cluster for visual affirmation of cell phenotypes.
No wash/no lyse leukocyte Examination with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Pro UX/UI enables buyers to teach AI types for expanded purposes. (A) The “Train Model” characteristic presents annotation tools and true-time product instruction accuracy.
At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which makes use of sound waves to meticulously align cells throughout the flow mobile. This modern technique surpasses standard hydrodynamic methods, leading to faster processing speeds, Remarkable facts good quality, and amplified sample throughput.
The Attune Xenith cytometer can be a superior-parameter spectral analyzer, delivering both spectral unmixing and standard flow abilities for complete and multipurpose cell Investigation.
Agilent Worth Promise ensures you not less than ten years of instrument use through the day you buy. Usually, We are going to credit history you While using the residual value of the system towards an upgraded design.
Right here, we clearly show that by gating techniques working with image-based mostly quantitative parameters can distinguish interacting cells from coincident situations to a lot more properly assess interacting cells.